Bag method & tissue culture question

[CactusChris]

Note entirely relevant but:

http://www.omnisterra.com/botany/cp/slides/tc/tc.htm

[Dirk Everaerd]

Have You try it this way:
[IMG]http://img117.imageshack.us/img117/6852/dsc01733xt8.jpg[/IMG]

Mama's old conservation pots, same way of the baggy-way for small amounts and when it is not enough to use the propagator. I use it for caudex-seeds!

[iann]

Cling film!
[img]http://www.galleries.thegardenforums.or ... eldii1.jpg[/img]

Not good for columnars though

[David_L]

Hi Nick

Try a quarter sized tray, my garden centre has these and they are available on the web. I get 4 species per tray, probably could get more. Ideally they should germinate and grow on at approximately the same rate. Don't mix Rebutias and Ariocarpus! I put 1 quarter sized tray in a medium freezer bag with a piece of stiff wire in the shape of a hoop to prevent the bag collapsing onto the compost and seal with a bag tie. Works fine.

[Julie]

A jam jar - great idea, Dirk!

Ian, does it keep the nasties out... does it occur to them that they get in through the drainage holes?

Cling film gives the best visibility, though!

Are those little lithops? They are the sweetest!

[Dirk Everaerd]

Not bad to read those:




Post Edited (12-20-06 22:45)

[iann]

Julie, I have to have visibility or I'll end up opening the baggies because I'm too nosy! I'm not too worried about what might get in through the drainage holes, its unlikely to cause mould or algae on the surface.

Yes, those are baby Lithops bromfieldii. They are one of the larger Lithops seeds but I still managed to get a few stuck together in the same spot even though I tried to place them one by one. I have a pot of L. hallii, perhaps the smallest Lithops seed and literally like fine dust. I just sprinkled them as best I could and they are nearly as well spaced as these

[Julie]

Ian, I am sure I would do the same! I'd have to peep!

Those are larger ones!?!?! How old are they, and how big will they be by the end of their first growing year?

I read somewhere that mixing fine grit with tiny seeds makes them easier to distribute, but.. don't know if that helps or whether it damages the seeds...